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λ dna hindiii digest dna ladder  (New England Biolabs)


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    Structured Review

    New England Biolabs λ dna hindiii digest dna ladder
    λ Dna Hindiii Digest Dna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 10752 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%BB+dna+hindiii+digest+ladder/HindIII/us12480151-211-14-21
    Average 99 stars, based on 10752 article reviews
    λ dna hindiii digest dna ladder - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: Development of a PCR-RFLP marker to genetically distinguish Prosorhynchus crucibulum and Prosorhynchus aculeatus.
    Article Snippet: Each reaction contained 1 standard unit Taq polymerase (Thermo Scientific), 5 μl PCR buffer 10×, 4 mMMgCl2, 0.2 mM μl dNTP (Roche), 200 nM of each primer, 5 and 10 μl template DNA of P. crucibulum and P. aculeatus, respectively, made up to 50 μl with water. .. This mix was placed in a ThermoCycler Primus 25/96 Hain Lifescience with the following conditions: initial hot start of 94 °C during 4 min followed by 40 cycles of 95 °C for 30 s, 40 °C for 30 s and 72 °C for 30 s. Final extension of the product was at 72 °C for 5 min. After amplification, the PCR products were checked by electrophoresis in a 1% agarose gel (BioRad) and fragment sizes were estimated using a λ DNA-HindIII digest ladder (New England BioLabs). .. For PCR product purification, a low melting 1% agarose gel was made (Promega) and a commercial kit (GFX PCR DNA and Gel band purification kit, GE Healthcare) was used according to the manufacturer's instructions.

    Polymerase Chain Reaction:

    Article Title: Development of a PCR-RFLP marker to genetically distinguish Prosorhynchus crucibulum and Prosorhynchus aculeatus.
    Article Snippet: Each reaction contained 1 standard unit Taq polymerase (Thermo Scientific), 5 μl PCR buffer 10×, 4 mMMgCl2, 0.2 mM μl dNTP (Roche), 200 nM of each primer, 5 and 10 μl template DNA of P. crucibulum and P. aculeatus, respectively, made up to 50 μl with water. .. This mix was placed in a ThermoCycler Primus 25/96 Hain Lifescience with the following conditions: initial hot start of 94 °C during 4 min followed by 40 cycles of 95 °C for 30 s, 40 °C for 30 s and 72 °C for 30 s. Final extension of the product was at 72 °C for 5 min. After amplification, the PCR products were checked by electrophoresis in a 1% agarose gel (BioRad) and fragment sizes were estimated using a λ DNA-HindIII digest ladder (New England BioLabs). .. For PCR product purification, a low melting 1% agarose gel was made (Promega) and a commercial kit (GFX PCR DNA and Gel band purification kit, GE Healthcare) was used according to the manufacturer's instructions.

    Electrophoresis:

    Article Title: Development of a PCR-RFLP marker to genetically distinguish Prosorhynchus crucibulum and Prosorhynchus aculeatus.
    Article Snippet: Each reaction contained 1 standard unit Taq polymerase (Thermo Scientific), 5 μl PCR buffer 10×, 4 mMMgCl2, 0.2 mM μl dNTP (Roche), 200 nM of each primer, 5 and 10 μl template DNA of P. crucibulum and P. aculeatus, respectively, made up to 50 μl with water. .. This mix was placed in a ThermoCycler Primus 25/96 Hain Lifescience with the following conditions: initial hot start of 94 °C during 4 min followed by 40 cycles of 95 °C for 30 s, 40 °C for 30 s and 72 °C for 30 s. Final extension of the product was at 72 °C for 5 min. After amplification, the PCR products were checked by electrophoresis in a 1% agarose gel (BioRad) and fragment sizes were estimated using a λ DNA-HindIII digest ladder (New England BioLabs). .. For PCR product purification, a low melting 1% agarose gel was made (Promega) and a commercial kit (GFX PCR DNA and Gel band purification kit, GE Healthcare) was used according to the manufacturer's instructions.

    Agarose Gel Electrophoresis:

    Article Title: Development of a PCR-RFLP marker to genetically distinguish Prosorhynchus crucibulum and Prosorhynchus aculeatus.
    Article Snippet: Each reaction contained 1 standard unit Taq polymerase (Thermo Scientific), 5 μl PCR buffer 10×, 4 mMMgCl2, 0.2 mM μl dNTP (Roche), 200 nM of each primer, 5 and 10 μl template DNA of P. crucibulum and P. aculeatus, respectively, made up to 50 μl with water. .. This mix was placed in a ThermoCycler Primus 25/96 Hain Lifescience with the following conditions: initial hot start of 94 °C during 4 min followed by 40 cycles of 95 °C for 30 s, 40 °C for 30 s and 72 °C for 30 s. Final extension of the product was at 72 °C for 5 min. After amplification, the PCR products were checked by electrophoresis in a 1% agarose gel (BioRad) and fragment sizes were estimated using a λ DNA-HindIII digest ladder (New England BioLabs). .. For PCR product purification, a low melting 1% agarose gel was made (Promega) and a commercial kit (GFX PCR DNA and Gel band purification kit, GE Healthcare) was used according to the manufacturer's instructions.

    Marker:

    Article Title: The TEL patch of telomere protein TPP1 mediates telomerase recruitment and processivity
    Article Snippet: .. A 5' 32 P-labeled (with T4 PNK; NEB) λ DNA-HindIII digest ladder (10,000 cpm) was run as a marker on a separate lane on the gel. ..



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